We are proud to contribute to the science of cosmetics by offering range of active, functional ingredients from stem cells.

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Stem cell derived Nanovesicles: The future of cruelty free cosmetics

Today, the mountains of beauty blogs out there are stating about the “active ingredients” like how these active ingredients address acne, hydration, as well as brightening concerns. However, nothing has been detailed about those active ingredients and how they can help in skin care.

Researchers have discovered that stem cell-derived exosomes and lysosomes are molecules secreted by stem cells; and are the mainstream agents that can talk to one another about aging, differentiation, stimulation, and secretion of anti-inflammatory factors that control and coordinate the process across the entire tissues in our body. In recent decades researchers have evolved their understanding of stem cell science and how to enhance their potential that may lead to longevity in the form of glowing skin, good hair, and growing old with a decreased likelihood of diseases and other health problems, i.e. growing older with grace.

	Active Ingredients	Role in cosmetic care
	Liposomes	Protects skin cells from external stressors Help in absorption of more active cosmetic ingredients Increase skin hydration through surface adhesiveness Increase performance of the base cosmetic products
	Niosomes	Can enhance sustainability and delivery of active ingredients Improve skin hydration, elasticity and smoothness Promote skin lightening
	Ethosomes	A very interesting and efficient delivery system to increase the resident time of cosmetic chemical at the deepest layer of the skin.
	Transferosomes	A biocompatible and biodegradable option to increase the delivery of cosmetically active components at the deepest layer of the skin. Increase skin flexibility, and hydration
	Exosomes	Promote intracellular communication Improve skin vascularization and reepithelization Promote reduced melanin production Help in minimizing scar formation Promote collagen synthesis

QUALITY

Optimal-Purity, Isolated Stem Cell Exosomes

INNOVATION

MSC Exosome Therapeutics and Diagnostics

REGULATION

CGMP Development of MSC Exosomes

Active Ingredients: The Next Big Thing in Cosmetic Industry?

These active functional components, isolated from mesenchymal stem cells including exosomes carry the legacy of stem cells in the form of their intrinsic, biological properties that can inhibit the same effect as stem cells do; thus, diverting ethical obligation of stem cells in skin care.

Anti-inflammatory

Downregulating inflammatory cytokines like TNF-alpha, IL-1 Beta, and MMPs (Matrix metalloproteinase), while upregulating inflammatory cytokines like IL-4, IL-10 and TIMPs. Thus, MSC derived exosomes can reduce inflammation due to skin ageing.

Anti-oxidant

The proteins like peroxiredoxins could reduce the oxidation state of cells under tremendous oxidative stress due to ageing. Thus, reducing oxidative stress on cells could improve their functions and survival.

Anti-apoptotic

These components are actively involved in maintaining cellular viabilities and preventing early cell death. The anti-apoptotic messengers these components are carrying along with the same is responsible for slow, stopping and/or reversing progression of ageing.

Anti-Fibrotic

These components including exosomes are associated with optimization of damaged skin tissues with improved collagen type I/III ratios and normal organization of collagen fibres.

Immunomodulatory

They are involved in regulation of immune cells like macrophages and T cells in different prototypes and are involved in activation of anti-inflammatory cytokines, preventing ageing.

Pro-Angiogenic

By stimulating endothelial cell function, these cells are involved in vascularization leading to tissue regeneration

Pro-synthetic

The pro-synthetic effects are helpful in activation of skin cells and enhance extracellular matrix production. Specific exosomes molecules are helpful in synthesis of collagen.

Epigenetics

Proteins that are present in these active ingredients are important for induction of inactive DNA transcription.

The fundamental purpose of Advancells is to be a part of the growing cosmetic industry by offering range of functionally active ingredients inheriting the power of stem cells.

Presently, cosmetic companies are using active ingredients from plants for the desired effects on skin care; however, it is very important to understand that skin care in our body is naturally controlled with the help of stem cells present within us. Due to ageing, these resident stem cells start losing their capacity to differentiate and regulate skin cells. Geriatric researchers have discovered that multiple factors are responsible for ageing, one of them is inflammatory role played by external stressors like pollution, diseased condition, diet etc.

We have identified the most potent sources of stem cells, isolated from Fetal tissues with in their naïve state. The active ingredients that we isolate from stem cells through unique technology are basically packed controlling codes, secreted by stem cells. Similar codes are inserted in other cells by hijacking them for the production of multiple proteins like collagen, secretion of anti-inflammatory markers for anti-ageing etc. Thus, these active ingredients open the miraculous way towards anti-ageing and longevity.These ingredients are being produced on a mass scale, quality assessed at each step of their development and before the release of the final batch.

Advancells has established an innovative expertise in cosmetic industry by harnessing active ingredients that can do wonders in skin care, hair care, sun care, make up and beauty from within. We present a unique formulation in the form of NANOREGEN complex, a synergistic blend of cutting-edge medical aesthetic treatment and cellular power performers, the mesenchymal stem cells and exosomes.

We are into designing, manufacturing and sourcing high-end cosmetically active functional ingredients. These bunch of breakthroughs in personalized cosmetic industry are well researched for cosmetic benefits and thoroughly tested for quality integrity. We want to be the most preferred partner of choice for offering them in cosmetic formulations.

The NanoRegen Complex

The NanoRegen Complex is an intensive dual-action complex formulated by combining the science of regenerative medicine with that of nanotechnology to support targeted delivery through quick absorption in the skin. The complex is concentrated using the power of over 3 billion stem cells derived exosomes; which are clinically proven to repair, regenerate and rejuvenate skin.

Exosomes And Skin

Exosomes, the emerging bioactive substances have been studied for a variety of skin conditions, including wound healing, hyperpigmentation, acne, dermatitis, and aging marks. They support to initiation of “cross-talk” between melanocytes, keratinocytes, and skin fibroblasts to promote the secretion of collagen, and hyaluronic acid which are important for modulating skin pigmentation and aging. Studies have further indicated that these mesenchymal stem cells-derived exosomes are classified as potential non-sensitizers and are completely safe for use without any adverse effects.

Exosomes And Hair

The role of exosomes in treating conditions like alopecia has also been proven in many recent clinical studies. The exosomes derived from mesenchymal stem cells with trans-differentiation potential, when injected into hair follicles at various stages of development can accelerate the onset of anagen, i.e. hair follicle strengthening, catagen i.e. apoptosis of hair follicles, and lengthening of hair shafts. Studies have also indicated that these exosomes can stimulate different growth factors to promote elongation of hair shafts and thus are promising candidates in the treatment and prevention of hair loss.

Exosomes And Longevity

Studies have indicated that exosomes, when isolated from mesenchymal stem cells that are known for their longevity can potentially reverse physiological markers of aging like impaired immune functions, and dysfunctional organs leading to alleviation of age-induced deficits in other cognitive functions.

ELISA TESTING

EXOSOMES COUNT

NANOREGEN COMPLEX

The NanoRegen Complex

We offer range of these active ingredients, isolated from a complex formed by nanotechnology and regenerative medicine. Owing to the various benefits of these active ingredients, isolated from stem cells; they are being widely used in various cosmetic applications. Their key benefits are higher stability, non-toxic and do not elicit any immune response; making them ideal candidates for personalized skin treatment products.

List of growth factors secreted by these active ingredients

The analysis of the secretome obtained from culturing mesenchymal stem cells in xenofree media, using Luminex multiplexing revealed the list of secreted growth factors. The cells were incubated in the media for 48 hrs.

	Growth Factors	Concentration (pg/ml)
AR	Androgen Receptors	235
BNF	Brain derived neurotropic factors	10
bFGF	Basic fibroblast growth factors	63
BMP-4	Bone morphogenetic protein-4	1
BMP-5	Bone morphogenetic protein-5	5660
NGF	Nerve growth factor	0
EGF	Epidermal growth factor	57
HGF	Hepatocyte growth factor	2425
IGF	Insulin like growth factor	67.5
PDGF	Platelet Derived Growth Factor	546
VEGF	Vascular Endothelial Growth Factor	2742
MCSF	Macrophage colony stimulating factor
TGF-α	Transforming growth factor-alpha	0
TGF-β	Transforming growth factor-beta	0
SCF	Stem cell factor	1500

Flow Cytometry Analysis/Western Blotting

The isolated exosomes were tested with surface markers specifically defined for exosomes like CD 63, CD 81 and CD 9; both using flow analysis and western blot techniques. We observed that each batch of exosome show 82% positivity towards CD 63, 89% positivity towards CD 81 and around 83% positivity towards CD9.

The western blot analysis was proposed to estimate total protein present in each batch of exosomes and was estimated to be around 6 ug/ml, as evident in the figures and graphs, mentioned herewith

FAQ

1. How are Exosomes infused into the body for various applications?

Exosomes can be infused locally via intrathecal, intranasal, intra-organ, or IV injection. Since exosomes are cell-free versions of stem cells carrying the same machinery as stem cells are; they can also be applied topically in case of skin ulcers, and wound regeneration. In the case of skin disorders, exosomes can directly infuse locally through a micro-needling system. With our advanced technology, we concentrate exosomes in a batch of 15 billion per ml.

2. What can I expect after the procedure? Are there any side effects?

The treatment is usually performed on an outpatient basis, generally requiring only local anesthesia. All patients should expect to get discharged with minimum downtime. We have studied that almost 90% of the patients getting treated with exosome/stem cell therapy did not report any major complications. While remaining 10% reported mild to moderate fever, nausea, lethargy, and vomiting post-infusion. At the same time, it should also be noted that symptoms cannot last for more than 24 hrs.

3. How long do Exosomes work in the body? What is the timeline?

The NanoRegen complex as discussed in our earlier sections generally triggers a so-called bi-phasic response. At first, immediate reactions are noted within 24 hrs of exosome/stem cell infusion, to initiate protein breakdown and release of messenger RNA. The messenger RNA i.e., responsible for hijacking other tissue-specific cells and resident stem cells can initiate a healing program. The said cascade of healing reactions lasts for about 8-10 weeks and the continued effect may be experienced for months afterward.

4. When can a person expect to see benefits?

During the initial few days post-therapy, patients were asked to continue with different rehabilitation programs like acupuncture, physiotherapy, hypothermia, etc. due to which he/she may report an immediate reduction of their inflammatory response. The following weeks can be accompanied by symptoms like loss of energy, excessive fatigue, often feeling thirsty/hungry, etc. due to activation of the cascade of healing and repairing processes. Some patients have also reported flare-ups of symptoms of their medical concerns due to reprogramming of the present system. Typically, early benefits can be observed within 3-4 months and continued for 9-12 months, post which a booster shot is needed.

5. Are exosomes safer than stem cells?

Both adult stem cells and their exosomes have definitive space in the healing mechanism; hence, a combinational approach is suggested for better clinical outcomes.